Publications

2012

Three-Dimensional Quantification of Cellular Traction Forces and Mechanosensing of Thin Substrata by Fourier Traction Force Microscopy, del Alamo, JC; Meili, R.; Alvarez-Gonzalez, B; Alonso-Latorre, B; Firtel, RA; Lasheras, JC. Submitted. (2012)

Three-dimensional deformation field (panels a-c) and stress field (panels d-f) generated by migrating Dictyostelium cell on the surface of the substratum

Flow of a viscous nematic fluid around a sphere, Gomez-Gonzalez, M; del Alamo JC. Submitted (2012).

Streamlines on the meridional and equatorial planes of a sphere moving perpendicular to the nematic director in nematic fluids with different viscosity ratios.

Main diastolic chamber properties of the left ventricle assessed by optimization fitting of pressure-volume data. An improved gold-standard of diastolic function. Bermejo, J; Yotti, R; Perez-del-Villar, C; Benito, Y; Antoranz, JC; Desco, MM; del Alamo, JC; Gonzalez-Mansilla, A; Rodriguez Perez, D; Barrrio, A; Martinez-Legazpi, P; Elizaga, J; Fernandez-Aviles, F. Submitted (2012).

Sensitivity analysis of LV main diastolic properties measured by the Jacobian of the LV pressure and the parametric sensitivity matrix.

Chemotaxing amoeboid cells migrate by switching between distinct adhesion dynamics and contractility modes. Bastounis, E; Meili, R; Alvarez-Gonzalez, B; del Alamo, JC; Firtel RA; Lasheras, JC. Submitted. (2012).

Tension kymograph illustrating different migration modes in a WT chemotaxing Dictyostelium cell

Dynamic and Reversible Surface Topography Influences Cell Morphology. Kiang, JD; del Alamo, JC; Engler, AJ. Submitted (2012).

Deformation caused in a soft gel embedded with magnetic microwires under magnetic field excitation.

In Situ Mechanotransduction via Vinculin Regulates Stem Cell Myogenesis. Holle, A; Tang, X; Vijayraghavan, D; Vincent, L; Fuhrmann, A; Choi, YS, del Alamo JC; Engler, AJ. Submitted (2012).

3D traction stresses generated by wild-type and vinculin knock-down human mesenchymal stem cells

Roles of cell confluency and fluid shear in 3-dimensional intracellular forces in endothelial cells. Hur, SS; del Alamo, JC; Park, JC; Li, YS; Nguyen, HA; Teng, D; Wang, KC; Flores, L; Alonso-Latorre, B; Lasheras, JC; Chien, S. Proceedings of the National Academy of Sciences of the USA 109 (28) 11110-11115 (2012).

3D traction stresses exerted by EC monolayers under constant laminar flow shear of 12 dyn/cm2 at time 0, 0.5 hours, and 24 hours. The white arrow indicates the flow direction. Scale bar is 10 m. Unit of color bar is Pa.

Commentary on our paper by Warren C. Ruder and Phillip R. Leduc.

Anisotropic rheology and directional mechanotransduction in vascular endothelial cells. del Alamo, J; Norwich, G; Li, Y; Lasheras, JC; Chien, S. Proceedings of the National Academy of Sciences of the United States of America, 105(40), 15411-15416. (2008).

Magnitude and directionality of the cytoplasmic shear modulus in vascular endothelial cells subjected to static conditions and laminar shear flow.

2008

Vorticity organization in the outer layer of turbulent channels with disturbed walls. Flores, O, Jimenez, J, & Del Alamo, J. Journal of fluid mechanics, 591, 145-154. (2007).

Isosurfaces of streamwise velocity fluctuations in smoothed-walled (top) rough-walled (bottom) turbulent channels, showing similar cone-shaped large structures.

Spatio-temporal analysis of eukaryotic cell motility by improved force cytometry. del Alamo; J, Meili, R; Alonso-Latorre, B; Rodriguez-Rodriguez, J; Aliseda, A; Firtel, R; Lasheras, JC. Proceedings of the National Academy of Sciences of the United States of America, 104(33), 13343-13348. (2007).

The speed of amoeboid cells (Y-axis) correlates inversely with the period of their motility cycle (X-axis).

2007

Estimation of turbulent convection velocities and corrections to Taylor's approximation. Del Alamo, J; Jimenez, J. Journal of fluid mechanics, 640, 5-26. (2009).

Spectral distribution of the convection velocity of the streamwise fluctuations in a turbulent channel and their energy density in the near-wall region.

Commentary on our paper by Parviz Moin.

Distribution of traction forces associated with shape changes during amoeboid cell migration. Alonso-Latorre, B.; Meili, R.; Bastounis, E.; del Alamo, J.C.; Firtel, R.; Lasheras, J.C.; Conf Proc IEEE Eng Med Biol Soc Volume: 2009 Pages: 3346-9 Published: (2009)

Distribution of traction stresses (top row) and F-actin (second row) corresponding to the 4 most prevalent modes of shape change during amoeboid cell migration (bottom histograms).

Recent Advances in the Application of Computational Mechanics to the Diagnosis and Treatment of Cardiovascular Disease. del Alamo, J; Marsden, A; Lasheras, J. Revista española de cardiología, 62(7), 781-805 (2009).

Phase Contrast MRI showing blood velocity vectors in the left ventricle during early diastole (left) late diastole (center) and systole (right)

Turbulence and Internal Waves in a Stably-Stratified Channel Flow, Garcia-Villalba, M. and del Alamo, J. C. High Performance Computing in Science and Engineering ’08 (Nagel, W. E., Kröner, D. B. and Resch, M. M. editors), vol. 5, 217-227 Springer (2009).

Instantaneous vertical velocity fluctuations in a vertical plane of neutrally (top) and stably (bottom) stratified turbulent channels.

2009

2010

From imaging to prediction: Emerging non-invasive methods in pediatric cardiology. Bazilevs, Y; del Alamo, JC; Humphrey, J. Progress in Pediatric Cardiology, 30(1-2), 81-89. (2010).

2D+t Doppler flow mapping in a normal left ventricle overlaid on a sequence of anatomical B-Mode apical long-axis images

The mechanics of the adhesive locomotion of terrestrial gastropods. Lai, J; del Alamo, J; Rodriguez-Rodriguez, J; Lasheras, JC. Journal of experimental biology, 213(22), 3920-3933. (2010).

Velocity field (mm/s) in the ventral surface of a banana slug moving from left to right.

Two-Dimensional Intraventricular Flow Mapping by Digital Processing Conventional Color-Doppler Echocardiography Images. Garcia, D; del Alamo, J; Tanne, D; Yotti, R; Cortina, C; Bertrand, E; Carlos Antoranz, J; Perez-David, E; Rieu, R; Fernandez-Aviles, F; Bermejo, J. IEEE transactions on medical imaging, 29(10), 1701-1713. (2010).

Blood velocity field (m/s) during late filling in a dilated ventricle.

Resolution and uniqueness of estimated parameters of a model of thin filament regulation in solution. Mijailovich, S; Li, X; del Alamo, J; Griffiths, R; Kecman, V; Geeves, M. Computational biology and chemistry, 34(1), 19-33 (2010).

Schematic representation of 3-state actomyosin conctraction model with tropomyosin - troponin repeating each 7 actin units.

Myosin II Is Essential for the Spatiotemporal Organization of Traction Forces during Cell Motility. Meili, R; Alonso-Latorre, B; del Alamo, J; Firtel, R; Lasheras, JC. Molecular biology of the cell, 21(3), 405-417. (2010).

Average traction stresses during different phases of the motility cycle in wild type amoeboid cells (top row), and in mutant cells lacking the myosin II essential light chain (center row) and the myosin II heavy chain (bottom row).

2011

Turbulence modification by stable stratification in channel flow. Garcia-Villalba, M; del Alamo, JC. Physics of Fluids vol 23, 045104 (2011).

Density fluctuations in the near-wall region of strongly stably stratified turbulent channels. Black boxes indicate the size of previous simulations.

The SCAR/WAVE complex is necessary for proper regulation of traction stresses during amoeboid motility. Bastounis, E.; Meili, R.; Alonso-Latorre, B.; del Alamo, JC; Lasheras, JC; Firtel, RA. Molecular Biology of the Cell 22 (21) 3995-40003 (2011).

Traction stresses exerted by chemotaxing wild-type Dictyostelium cells and mutants with enhanced and decreased SCAR/WAVE activation.

An Oscillatory Contractile Pole-Force Component Dominates the Traction Forces Exerted by Migrating Amoeboid Cells. Alonso-Latorre, B.; del Alamo, JC; Meili, R; Firtel, RA; Lasheras, JC. Cellular and Molecular Bioengineering 4 (4), 603-615 (2011).

Traction stress map corresponding to the dominant motility mode in wild type Dictyostelium cells, and in mutants with contractility and cross-linking defects.

2004

The large-scale dynamics of near-wall turbulence. Jimenez, J; Del Alamo, J; Flores, O. Journal of fluid mechanics, 505, 179-199. (2004).

Structure of the streamwise (top) and wall-normal (bottom) turbulent fluctuations in an autonomous wall region.

Scaling of the energy spectra of turbulent channels. Del Alamo, J; Jimenez, J; Zandonade, P; Moser, R. Journal of fluid mechanics, 500, 135-144. (2004).

Scaling of the streamwise (left panel, lines) and wall-normal (left panel, symbols) turbulent spectra in the logarithmic region, and conceptual model for this scaling (right panel).

2006

Self-similar vortex clusters in the turbulent logarithmic region. del Alamo, J; Jimenez, J; Zandonade, P; Moser, R. Journal of fluid mechanics, 561, 329-358. (2006).

Example of wall-attached vortex cluster in a turbulent channel.

Linear energy amplification in turbulent channels. del Alamo, J; Jimenez, J. Journal of fluid mechanics, 559, 205-213. (2006).

Maximal energy amplification of infinitely long streamwise fluctuations as a function of their width. The first peak predicts the distance between near-wall streaks (100+) while the second peak predicts the width of the large structures in the outer region of the flow.

Spectra of the very large anisotropic scales in turbulent channels. del Alamo, J; Jimenez, J. Physics of fluids, 15(6), L41-L44. (2003).

Spectrograms (wavelength - wall distance) of the streamwise fluctuations in a turbulent channel. Top panels: scales larger than half channel height. Bottom panels: scales smaller than half channel heigh.

2003